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TESTING SERVICES

 Apex Biotechnology Training and Research Institute extended their expertise and instrumentation testing facility to primarily help the students, researchers, scientists, entrepreneurs, and corporate partners. Apex Biotechnology Training and Research Institute aim at providing more precise and reproducible results on any given samples by in-time frame. At most all advanced biotechnological analyses have been covered with the state-of-the-art instrumentation and cutting edge technologies with affordable cost. 

MOLECULAR IDENTIFICATION OF ORGANISMS

  • Bacterial identification
  • Fungal Identification
  • Plants Identification
  • Animal Identification

Molecular techniques and Recombinant Technology

  •  Polymerase Chain Reaction
  • Cloning, Vector Construction and Expression
  • Restriction Fragment Length Polymorphisms (RFLP)
  • Amplified Fragment Length Polymorphisms (AFLP)
  • Amplified Ribosomal DNA Restriction Analysis (ARDRA)
  • Random Amplification of Polymorphic DNA (RAPD)
  • cDNA Library Construction
  • Competent Cell Preparation
  • Total DNA/RNA Isolation
  • Gel Electrophoresis (SDS-PAGE, Native PAGE, Agarose)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot analysis
  • Real-Time PCR Analysis
  • 2D Protein Gel Electrophoresis
  • DNA Sequencing Services
  • Zymogram analysis

In-vitro Bioassays

  • Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoan, Antialgal, Anticandidal)
  • Anticancer activity (viz., Cell lines, Cytotoxicity, MTT assay, DNA fragmentation, Apoptotic Protein expression)
  • Antioxidant activity (DPPH scavenging, ABTS scavenging, FRAP, TRAP, Reducing power, etc.)
  • Antiinflammatory, Antilipidimic, Antidiabetic activity, Enzyme Inhibition
  • Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics, Enzyme applications).

Phytochemistry

  • Phytochemical Screening (qualitative)
  • Phytochemical quantification.
  • Vitamins and minerals content.
  • Nutritional analysis.

Purification of Active molecules and Enzymes

  • Bioactive compound purification from Bacteria, Actinobacteria, Fungi, Algae, Medicinal Plants, Animals sources
  • Extracellular or Intra-cellular metabolites purification
  • Silica Gel Column Chromatography (Low/High resolution) for Secondary metabolite
  • Neutral Alumina column chromatography
  • Sephadex LH20 Column Chromatography for Secondary metabolites
  • Ion Exchange Column Chromatography form Protein/Carbohydrates
  • Size Exclusion Column Chromatography (Gel Permeation Column Chromatography)
  • Affinity Column Chromatography
  • Fast Protein Liquid Chromatography
  • Thin Layer Chromatography (TLC); High Performance Thin Layer Chromatography (HPTLC)
  • Gas Chromatography (GC)
  • Preparative High Performance Liquid Chromatography

Spectral Analyses and Secondary Metabolites Structure Elucidation

 

  • UV-Vis Spectrometry (UV-Vis spectrum).
  • Infra Red spectrum (FT-IR, KBr pellet, UV-Vis-NIR).
  • NMR spectrum (HNMR, CNMR, 2D NMR).
  • Mass Spectrum (GC-MS, ISI-MS, Maldi-Tof MS, LC-MS, LC_MS/MS).
  • High Performance Liquid Chromatography (HPLC).
  • High Performance Thin Layer Chromatography (HPTLC).
  • X-ray Diffraction (Single crystal XRD / Powder XRD).
  • Atomic absorption Spectorscopy (AAS) and CHNSO Analysis.
  • Inductively coupled plasma mass spectrometry (ICP-MS) .
  • Electron Microscopy (HRSEM, HRTEM, AFM, Phase Contrast) Analysis.
  • Molecular structure elucidation.

Other Instrumentation


  • Lyophilization
  • Muffle Furnace
  • Rotoevaporator
  • Microplate Reader
  • CO2 incubator
  • Western Blot
  • Ettan IPGPhore 2D gel System
  • Real-Time PCR
  • Fluorescence Microscope
  • Conventional PCR

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Downloads the Price List 2019 & Request Form

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