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TESTING SERVICES

Apex Biotechnology Training and Research Institute extended their expertise and instrumentation testing facility to primarily help the students, researchers, scientists, entrepreneurs, and corporate partners. Apex Biotechnology Training and Research Institute aim at providing more precise and reproducible results on any given samples by in-time frame. At most all advanced biotechnological analyses have been covered with the state-of-the-art instrumentation and cutting edge technologies with affordable cost.

mOLECULAR iDENTIFICATION OF ORGANISMS

▶ Bacterial identification

▶ Fungal Identification

▶ Plants Identification

▶ Animal Identification

Molecular techniques and Recombinant Technology

▶ Polymerase Chain Reaction

▶ Cloning, Vector Construction and Expression

▶ Restriction Fragment Length Polymorphisms (RFLP)

▶ Amplified Fragment Length Polymorphisms (AFLP)

▶ Amplified Ribosomal DNA Restriction Analysis (ARDRA)

▶ Random Amplification of Polymorphic DNA (RAPD)

▶ cDNA Library Construction

▶ Competent Cell Preparation

▶ Total DNA/RNA Isolation

▶ Gel Electrophoresis (SDS-PAGE, Native PAGE, Agarose)

▶ Enzyme-Linked Immunosorbent Assay (ELISA)

▶ Western Blot analysis

▶ Real-Time PCR Analysis

▶ 2D Protein Gel Electrophoresis

▶ DNA Sequencing Services

▶ Zymogram analysis

In-vitro Bioassays

Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoan, Antialgal, Anticandidal)

Anticancer activity (viz., Cell lines, Cytotoxicity, MTT assay, DNA fragmentation, Apoptotic Protein expression)

Antioxidant activity (DPPH scavenging, ABTS scavenging, FRAP, TRAP, Reducing power, etc.)

Antiinflammatory, Antilipidimic, Antidiabetic activity, Enzyme Inhibition

Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics, Enzyme applications).

Spectral Analyses and Secondary Metabolites Structure Elucidation

UV-Vis Spectrometry (UV-Vis spectrum).

Infra Red spectrum (FT-IR, KBr pellet, UV-Vis-NIR).

NMR spectrum (HNMR, CNMR, 2D NMR).

Mass Spectrum (GC-MS, ESI-MS, Maldi-Tof MS, LC-MS, LC_MS/MS).

High Performance Liquid Chromatography (HPLC).

High Performance Thin Layer Chromatography (HPTLC).

X-ray Diffraction (Single crystal XRD / Powder XRD).

Atomic absorption Spectroscopy (AAS) and CHNSO Analysis.

Inductively coupled plasma mass spectrometry (ICP-MS) .

Electron Microscopy (HRSEM / HRTEM) Analysis.

Molecular Structure Elucidation.

Purification of Active molecules and Enzymes

Bioactive compound purification from Bacteria, Actinobacteria, Fungi, Algae, Medicinal Plants, Animals sources

Extracellular or Intra-cellular metabolites purification

Silica Gel Column Chromatography (Low/High resolution) for Secondary metabolite

Neutral Alumina column chromatography

Sephadex LH20 Column Chromatography for Secondary metabolites

Ion Exchange Column Chromatography form Protein/Carbohydrates

Size Exclusion Column Chromatography (Gel Permeation Column Chromatography)

Affinity Column Chromatography

Fast Protein Liquid Chromatography

Thin Layer Chromatography (TLC); High Performance Thin Layer Chromatography (HPTLC)

Gas Chromatography (GC)

Preparative High Performance Liquid Chromatography

Phytochemistry

Phytochemical Extraction

Phytochemical Screening (Qualitative)

Phytochemical Screening (Quantitative)

Vitamins and minerals content.

Nutritional analysis.

Other Instrumentation

Lyophilizer - Bench Top

Muffle Furnace

Roto-evaporator

Microplate Reader

CO2 incubator

Western Blot

Ettan IPGPhore 2D gel System

Real-Time PCR

Fluorescence Microscope

Gradient PCR

Trinocular Phase Contrast Microscope

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