TESTING SERVICES

Apex Biotechnology Training and Research Institute extends its expertise and instrumentation testing facilities to support students, researchers, scientists, entrepreneurs, and corporate partners. We aim to deliver precise and reproducible results within a timely frame. Our services cover a wide range of advanced biotechnological analyses using state-of-the-art instrumentation and cutting-edge technologies, all at affordable costs.

mOLECULAR iDENTIFICATION OF ORGANISMS

▶ Bacterial identification

▶ Fungal Identification

▶ Plants Identification

▶ Animal Identification

Molecular techniques and Recombinant Technology

▶ Polymerase Chain Reaction

▶ Cloning, Vector Construction and Expression

▶ Restriction Fragment Length Polymorphisms (RFLP)

▶ Amplified Fragment Length Polymorphisms (AFLP)

▶ Amplified Ribosomal DNA Restriction Analysis (ARDRA)

▶ Random Amplification of Polymorphic DNA (RAPD)

▶ cDNA Library Construction

▶ Competent Cell Preparation

▶ Total DNA/RNA Isolation

▶ Gel Electrophoresis (SDS-PAGE, Native PAGE, Agarose)

▶ Enzyme-Linked Immunosorbent Assay (ELISA)

▶ Western Blot analysis

▶ Real-Time PCR Analysis

▶ 2D Protein Gel Electrophoresis

▶ DNA Sequencing Services

▶ Zymogram analysis

In-vitro Bioassays

▶ Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoan, Antialgal, Anticandidal)

▶ Anticancer activity (viz., Cell lines, Cytotoxicity, MTT assay, DNA fragmentation, Apoptotic Protein expression)

▶ Antioxidant activity (DPPH scavenging, ABTS scavenging, FRAP, TRAP, Reducing power, etc.)

▶ Antiinflammatory, Antilipidimic, Antidiabetic activity, Enzyme Inhibition

▶ Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics, Enzyme applications).

Spectral Analyses and Secondary Metabolites Structure Elucidation

▶ UV-Vis Spectrometry (UV-Vis spectrum).

▶ Infra Red spectrum (FT-IR, KBr pellet, UV-Vis-NIR).

▶ NMR spectrum (HNMR, CNMR, 2D NMR).

▶ Mass Spectrum (GC-MS, ESI-MS, Maldi-Tof MS, LC-MS, LC_MS/MS).

▶ High Performance Liquid Chromatography (HPLC).

▶ High Performance Thin Layer Chromatography (HPTLC).

▶ X-ray Diffraction (Single crystal XRD / Powder XRD).

▶ Atomic absorption Spectroscopy (AAS) and CHNSO Analysis.

▶ Inductively coupled plasma mass spectrometry (ICP-MS) .

▶ Electron Microscopy (HRSEM / HRTEM) Analysis.

▶ Molecular Structure Elucidation.

Purification of Active molecules and Enzymes

▶ Bioactive compound purification from Bacteria, Actinobacteria, Fungi, Algae, Medicinal Plants, Animals sources

▶ Extracellular or Intra-cellular metabolites purification

▶ Silica Gel Column Chromatography (Low/High resolution) for Secondary metabolite

▶ Neutral Alumina column chromatography

▶ Sephadex LH20 Column Chromatography for Secondary metabolites

▶ Ion Exchange Column Chromatography form Protein/Carbohydrates

▶ Size Exclusion Column Chromatography (Gel Permeation Column Chromatography)

▶ Affinity Column Chromatography

▶ Fast Protein Liquid Chromatography

▶ Thin Layer Chromatography (TLC); High Performance Thin Layer Chromatography (HPTLC)

▶ Gas Chromatography (GC)

▶ Preparative High Performance Liquid Chromatography

Phytochemistry

▶ Phytochemical Extraction

▶ Phytochemical Screening (Qualitative)

▶ Phytochemical Screening (Quantitative)

▶ Vitamins and minerals content.

▶ Nutritional analysis.

Other Instrumentation

▶ Lyophilizer - Bench Top

▶ Muffle Furnace

▶ Roto-evaporator

▶ Microplate Reader

▶ CO2 incubator

▶ Western Blot

▶ Ettan IPGPhore 2D gel System

▶ Real-Time PCR

▶ Fluorescence Microscope

▶ Gradient PCR

▶ Trinocular Phase Contrast Microscope