TESTING SERVICES

Apex Biotechnology Training and Research Institute extended their expertise and instrumentation facility to primarily help the students/researcher/scientists/entrepreneurs and industries. Apex Biotechnology Training and Research Institute aim at providing more precise and reproducible results on any given samples by in-time frame. At most all biotechnological analyses have been covered with the state-of-the-art instrumentations and cutting edge technologies with affordable cost.

Analysis Price List 2018

Sample submission Form

MOLECULAR IDENTIFICATION OF ORGANISMS

  • Bacterial identification

  • Fungal Identification

  • Plants Identification

  • Animal Identification

Molecular techniques and Recombinant Technology

  • Polymerase Chain Reaction

  • Cloning, Vector Construction and Expression

  • Restriction Fragment Length Polymorphisms (RFLP)

  • Amplified Fragment Length Polymorphisms (AFLP)

  • Amplified Ribosomal DNA Restriction Analysis (ARDRA)

  • Random Amplification of Polymorphic DNA (RAPD)

  • cDNA Library Construction

  • Competent Cell Preparation

  • Total DNA/RNA Isolation

  • Gel Electrophoresis (SDS-PAGE, Native PAGE, Agarose)

  • Enzyme-Linked Immunosorbent Assay (ELISA)

  • Western Blot analysis

  • Real-Time PCR Analysis.

  • 2D Protein Gel Electrophoresis

  • DNA Sequencing Services

In vitro Bioassays

  • Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoan, Antialgal, Anticandidal)

  • Anticancer activity (viz., Cell lines, Cytotoxicity, MTT assay, DNA fragmentation, Apoptotic Protein expression)

  • Antioxidant activity (DPPH scavenging, ABTS scavenging, FRAP, TRAP, Reducing power, etc.)

  • Antiinflammatory, Antilipidimic, Antidiabetic activity, Enzyme Inhibition

  • Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics, Enzyme applications).

Phytochemistry

  • Phytochemical Screening (qualitative)

  • Phytochemical quantification.

  • Vitamins and minerals content.

  • Nutritional analysis.

Purification of Active molecules and Enzymes

  • Bioactive compound purification from Bacteria, Actinomycetes, Fungi, Algae, Medicinal Plants, Animals sources

  • Extracellular or Intracellular metabolites purification

  • Silica Gel Column Chromatography (Low/High resolution) for Secondary metabolites

  • Sephadex LH20 Column Chromatography for Secondary metabolites

  • Ion Exchange Column Chromatography form Protein/Carbohydrates

  • Size Exclusion Column Chromatography (Gel Permeation Column Chromatography)

  • Affinity Column Chromatography

  • Fast Protein Liquid Chromatography

  • Thin Layer Chromatography (TLC); High Performance Thin Layer Chromatography (HPTLC)

  • Gas Chromatography (GC).

  • Preparative High Performance Liquid Chromatography.

Spectral Analyses and Secondary Metabolites Structure Elucidation

  • UV-Vis Spectrometry (UV-Vis spectrum).

  • Infra Red spectrum (FT-IR, KBr pellet, UV-Vis-NIR).

  • NMR spectrum (HNMR, CNMR, 2D NMR).

  • Mass Spectrum (GC-MS, ISI-MS, Maldi-Tof MS, LC-MS, LC_MS/MS).

  • High Performance Liquid Chromatography (HPLC).

  • High Performance Thin Layer Chromatography (HPTLC).

  • X-ray Diffraction (Single crystal XRD / Powder XRD).

  • Atomic absorption Spectorscopy (AAS) and CHNSO Analysis.

  • Inductively coupled plasma mass spectrometry (ICP-MS) .

  • Electron Microscopy (HRSEM, HRTEM, AFM, Phase Contrast) Analysis.

  • Molecular structure elucidation.

Other Instrumentation

  • Lyophilization

  • Muffle Furnace

  • Rotoevaporator

  • Microplate Reader

  • CO2 incubator

  • Western Blot

  • Ettan IPGPhore 2D gel System

  • Real-Time PCR

  • Fluorescence Microscope

  • Conventional PCR

Upcoming Programs